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pgl3 basic  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 51 article reviews
    pgl3 basic - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII. ..

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (-153 to -1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Plasmid Preparation:

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII. ..

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (-153 to -1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Binding Assay:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    Construct:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc.
    Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc

    other:

    Article Title: E2F1 suppresses Epstein-Barr virus lytic reactivation through cellular and viral transcriptional networks
    Article Snippet: pGL3 Basic Vector , Gift from Debrya Groskreutz , RRID: Addgene_212936.

    Luciferase:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Reporter Assay:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..



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