Review



pgl3 basic  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 49 article reviews
    pgl3 basic - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII. ..

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (-153 to -1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Plasmid Preparation:

    Article Title: Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 Regulation
    Article Snippet: The 519 bp ADTRP regulatory region flanking rs6903956 (-789 bp to +724 bp) (chr6:11,774,037–11,774,555) from Luo et al. was cloned into the pGL3 Control Vector (Addgene, Plasmid #212937) using KpnI and XhoI, upstream of the SV40 promoter and firefly luciferase coding region. .. Additionally, the same 519 bp region was cloned into the pGL3 Basic Vector (Addgene, Plasmid #212936) using XhoI and HindIII. ..

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (-153 to -1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: A Chemiluminescent EMSA kit (Beyotime #GS009) was used to detect interactions between the probes and nuclear proteins extracted from control and UHRF1 -knockout A549 cells using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Thermo #78833). .. The APN promoter sequences (−153 to −1) were cloned into the pGL3 Basic vector (Addgene ##212936), resulting in the plasmid pGL3-APN-Luc. .. The pGL3-APN-Luc plasmid was methylated in vitro using CpG Methyltransferase M.SssI (NEB #M0226S).

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Binding Assay:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Construct:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    other:

    Article Title: E2F1 suppresses Epstein-Barr virus lytic reactivation through cellular and viral transcriptional networks
    Article Snippet: pGL3 Basic Vector , Gift from Debrya Groskreutz , RRID: Addgene_212936.

    Luciferase:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Reporter Assay:

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Article Title: ACTL6A depletion induces KLF4-mediated anti-tumorigenic effects in colorectal cancer.
    Article Snippet: .. The pGL3 basic vector (#212936; Addgene) was used for the luciferase reporter assay. ..

    Transfection:

    Article Title: Zinc finger protein 184 prevents α-synuclein preformed fibril-mediated neurodegeneration through the interleukin enhancer binding factor 3-microRNA-7 pathway.
    Article Snippet: .. SH-SY5Y cells were transfected for 48 h using the pGL3-basic vector (Addgene, Watertown, MA, USA), pGL3-human ILF3 promoter (pGL3-hILF3), Flag-ZNF184, pGL3-MT1, pGL3-MT2, and pGL3-MT1/2 in Opti-MEM (Gibco, Grand Island, NY, USA) with the Xtreme-gene (Roche Holding AG, Basel, Switzerland). .. First, the cells were lysed using a passive lysis buffer, and then luciferin, Stop&Glo substrate, and buffer from a dual-luciferase reporter assay kit (Promega, Madison, WI, USA) were added to the samples.



    Similar Products

    93
    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 1 article reviews
    pgl3 basic - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pgl3 basic vector
    Pgl3 Basic Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pmc12893712-79-26-28
    Average 93 stars, based on 1 article reviews
    pgl3 basic vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Addgene inc vector pgl3
    Vector Pgl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pmc12595211-52-8-6
    Average 93 stars, based on 1 article reviews
    vector pgl3 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    86
    Genechem pgl3 basic luciferase reporter vectors
    Pgl3 Basic Luciferase Reporter Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/abcb11+construct+gv238+luciferase+promoter+reporter/pmc12622415-72-22-26
    Average 86 stars, based on 1 article reviews
    pgl3 basic luciferase reporter vectors - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results